Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (5): 465-474.DOI: 10.16819/j.1001-7216.2017.6170 465
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Hong WANG, Yingxin ZHANG, Lianping SUN, Shuai MENG, Peng XU, Weixun WU, Shihua CHENG, Liyong CAO*(), Xihong SHEN*()
Received:
2016-12-23
Revised:
2017-02-08
Online:
2017-10-10
Published:
2017-09-10
Contact:
Liyong CAO, Xihong SHEN
王宏, 张迎信, 孙廉平, 孟帅, 徐鹏, 吴玮勋, 程式华, 曹立勇*(), 沈希宏*()
通讯作者:
曹立勇,沈希宏
基金资助:
CLC Number:
Hong WANG, Yingxin ZHANG, Lianping SUN, Shuai MENG, Peng XU, Weixun WU, Shihua CHENG, Liyong CAO, Xihong SHEN. Map-Based Cloning of OsCAD2 Regulating Golden Hull and Internode in Rice[J]. Chinese Journal OF Rice Science, 2017, 31(5): 465-474.
王宏, 张迎信, 孙廉平, 孟帅, 徐鹏, 吴玮勋, 程式华, 曹立勇, 沈希宏. 控制水稻金黄色颖壳与节间基因OsCAD2的图位克隆[J]. 中国水稻科学, 2017, 31(5): 465-474.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.6170 465
引物名称 Primer name | 上游引物 Forward primer(5′-3′) | 下游引物 Reverse primer(5′-3′) | 用途 Purpose |
---|---|---|---|
FH-1 | TTACCATATAGTATAGCTGGTTGG | AACGATTTAGAAGTCAAATTAAAA | 精细定位 Fine mapping |
FH-5 | ATTTTGATAATGGATAGAGATATTG | CACTTATAAATATAGAGAACGGATCT | 精细定位 Fine mapping |
FH-13 | ATTTGGTTACATCCGCCTTT | CAGTTCAGCCGTCCAATAGA | 精细定位 Fine mapping |
RH-1 | AGGAGCCTTGGATTACTACGA | GTTCACTGCCATGAACACATG | 精细定位 Fine mapping |
RH-4 | GAGAAGAGCACAAACAATCACA | GTTTTTCGGTGTCTCTCAACTA | 精细定位 Fine mapping |
RH-7 | TGCCAAACATATCGTGTCAC | ATTCCAAGCCCACTTCTTAA | 精细定位 Fine mapping |
RH-10 | TGAAACTGTTTTAAACTAGATTCG | TGCTGAAAAGACTACAAAGATATG | 精细定位 Fine mapping |
RH-21 | TTGATTGATTTTTTTCGATACAC | GGATTGTTAACTTTTAAAACTTCTG | 精细定位 Fine mapping |
RH-25 | TATGTGACTTGTATGTGTCCAGC | TTCCTATCATTTGATTCATTGTT | 精细定位 Fine mapping |
SE-5 | GGTGTAGACGTCGTTGTAGG | AGATGGATCATGGCTCATG | 测序 Sequencing |
Os4CL3 | CCAGCAGGTGGATGGAGAG | AGCACGGAGTTGAGCGAGTA | qRT-PCR |
OsMYB48 | TGTATGGGTGGTGTGTGGCT | CAGGAATGCTCTTGGCGA | qRT-PCR |
Table 1 Primers used in this study.
引物名称 Primer name | 上游引物 Forward primer(5′-3′) | 下游引物 Reverse primer(5′-3′) | 用途 Purpose |
---|---|---|---|
FH-1 | TTACCATATAGTATAGCTGGTTGG | AACGATTTAGAAGTCAAATTAAAA | 精细定位 Fine mapping |
FH-5 | ATTTTGATAATGGATAGAGATATTG | CACTTATAAATATAGAGAACGGATCT | 精细定位 Fine mapping |
FH-13 | ATTTGGTTACATCCGCCTTT | CAGTTCAGCCGTCCAATAGA | 精细定位 Fine mapping |
RH-1 | AGGAGCCTTGGATTACTACGA | GTTCACTGCCATGAACACATG | 精细定位 Fine mapping |
RH-4 | GAGAAGAGCACAAACAATCACA | GTTTTTCGGTGTCTCTCAACTA | 精细定位 Fine mapping |
RH-7 | TGCCAAACATATCGTGTCAC | ATTCCAAGCCCACTTCTTAA | 精细定位 Fine mapping |
RH-10 | TGAAACTGTTTTAAACTAGATTCG | TGCTGAAAAGACTACAAAGATATG | 精细定位 Fine mapping |
RH-21 | TTGATTGATTTTTTTCGATACAC | GGATTGTTAACTTTTAAAACTTCTG | 精细定位 Fine mapping |
RH-25 | TATGTGACTTGTATGTGTCCAGC | TTCCTATCATTTGATTCATTGTT | 精细定位 Fine mapping |
SE-5 | GGTGTAGACGTCGTTGTAGG | AGATGGATCATGGCTCATG | 测序 Sequencing |
Os4CL3 | CCAGCAGGTGGATGGAGAG | AGCACGGAGTTGAGCGAGTA | qRT-PCR |
OsMYB48 | TGTATGGGTGGTGTGTGGCT | CAGGAATGCTCTTGGCGA | qRT-PCR |
性状 Trait | WT | gh881 | P | |
---|---|---|---|---|
株高 Plant height/cm | 74.75±0.96 | 72.80±1.62 | **P<0.01 | |
单株有效穗数 No. of panicles per plant | 11.20±1.69 | 12.40±1.43 | P=0.07 | |
每穗总粒数 No. of spikelets per panicle | 132.31±6.19 | 104.03±8.08 | **P<0.01 | |
每穗实粒数 No. of grains per panicle | 117.46±4.94 | 65.31±10.99 | **P<0.01 | |
结实率 Seed-setting rate/% | 88.82±2.15 | 62.87±9.71 | **P<0.01 | |
千粒重 1000-grain weight/g | 28.92±0.51 | 27.13±1.12 | **P<0.01 |
Table 2 Comparison of main agronomic traits between gh881 and its wild type(WT)(Mean±SD, n=10).
性状 Trait | WT | gh881 | P | |
---|---|---|---|---|
株高 Plant height/cm | 74.75±0.96 | 72.80±1.62 | **P<0.01 | |
单株有效穗数 No. of panicles per plant | 11.20±1.69 | 12.40±1.43 | P=0.07 | |
每穗总粒数 No. of spikelets per panicle | 132.31±6.19 | 104.03±8.08 | **P<0.01 | |
每穗实粒数 No. of grains per panicle | 117.46±4.94 | 65.31±10.99 | **P<0.01 | |
结实率 Seed-setting rate/% | 88.82±2.15 | 62.87±9.71 | **P<0.01 | |
千粒重 1000-grain weight/g | 28.92±0.51 | 27.13±1.12 | **P<0.01 |
杂交组合 Cross combination | F1表型 F1 phenotype | F2分离比 Segregation in F2 | 总数 Total | χ23:1 | |
---|---|---|---|---|---|
野生型表型 Wild-type | 突变体表型 Mutant-type | ||||
gh881×中恢8015 gh881×Zhonghui 8015 | 正常Normal | 764 | 236 | 1000 | 1.05 |
gh881×长粒粳 gh881×Changlijing | 正常Normal | 756 | 244 | 1000 | 0.19 |
Table 3 Segregation of gh881 locus.
杂交组合 Cross combination | F1表型 F1 phenotype | F2分离比 Segregation in F2 | 总数 Total | χ23:1 | |
---|---|---|---|---|---|
野生型表型 Wild-type | 突变体表型 Mutant-type | ||||
gh881×中恢8015 gh881×Zhonghui 8015 | 正常Normal | 764 | 236 | 1000 | 1.05 |
gh881×长粒粳 gh881×Changlijing | 正常Normal | 756 | 244 | 1000 | 0.19 |
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